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建立一种检测Ｋｉ－ｒａｓ基因突变的快速、高敏感度的方法，并将其应用于大肠癌的检测中。应用引物诱导限制富集突变等位基因检测法（ＰＣＲ－ＰＩＲＥＭＡ，ＰｒｉｍｅｒＩｎｔｒｏｄｕｃｅｄＲｅｓｔｒｉｃｔｉｏｎｗｉｔｈＥｎ－ｒｉｃｈｍｅｎｔｏｆＭｕｔａｎｔＡｌｅｌｅＡｓｓａｙ）的敏感度达１０－５～１０－６，并在３０例大肠癌样品中检测出了８例Ｋｉ－ｒａｓ基因１２密码子突变的样本，突变率达２６．７％。证明ＲＣＲ－ＰＩＲＥＭＡ法是一种快速、敏感度高的方法，适用于在大量野生型拷贝中检测稀有的突变，可用于已知点突变的肿瘤的早期诊断及基因突变机制的动力学研究等许多方面
Establish a rapid, highly sensitive method for detecting Ki-ras mutations and apply them to the detection of colorectal cancer. The sensitivity of PCR-PIREMA (Primer Introduced Restriction with En-richment of Mutant AleleAssay) was 10-5 to 10-6, and 8 Ki-ras genes were detected in 30 colorectal cancer samples. In the sample with 12 codon mutations, the mutation rate was 26.7%. The RCR-PIREMA method is proved to be a rapid and highly sensitive method suitable for detecting rare mutations in a large number of wild-type copies. It can be used for the early diagnosis of tumors with known point mutations and the dynamics of gene mutation mechanisms. aspect