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目的 :探讨血管成形术后Ⅰ型胶原的动态演变规律及其与金属蛋白酶 1(MMP 1)和金属蛋白酶的组织型抑制剂 (TIMP 1)的关系。方法 :采用小型猪髂动脉再狭窄动物模型 ,分别在血管成形术后 1、2、3和 6个月取材 ,用免疫组化、透射电镜加图像分析技术观察血管内膜增生、平滑肌细胞 (VSMC)表型特征、Ⅰ型胶原、MMP 1和TIMP 1的表达情况。结果 :血管内膜增生及影像学狭窄在成形术后 3个月达高峰 ,Ⅰ型胶原的表达水平在成形术后逐渐增高 ,MMP 1的表达水平在血管成形术后早期较低 ,至 6个月时达正常血管水平 ,而TIMP 1在血管成形术后早期即明显增高 ,在 3个月内达高峰 ,6个月时仍稳定在较高水平 ;VSMC表型在早期主要是合成型 ,而后期以收缩型为主 ;TIMP 1/MMP 1的比值与新生内膜面积及Ⅰ型胶原的表达水平均呈显著正相关 (r=0 .6 5 ,P <0 .0 1)。结论 :血管成形术后细胞外Ⅰ型胶原随着时间的推移而逐渐增加 ,Ⅰ型胶原的动态演变主要取决于TIMP 1/MMP 1的活性比值 ,并与VSMC表型特征有一定的联系
Objective: To investigate the dynamic evolution of type I collagen after angioplasty and its relationship with the expression of metalloproteinase-1 and tissue inhibitor of metalloproteinase (TIMP-1). Methods: The animal models of mini-iliac artery restenosis were used in the study. The rats were drawn at 1, 2, 3 and 6 months after angioplasty. The expressions of vascular endothelial growth factor (VSMC) and vascular smooth muscle cells (VSMCs) were observed by immunohistochemistry and transmission electron microscope ) Phenotype, type I collagen, MMP 1 and TIMP 1 expression. Results: Intimal hyperplasia and imaging stenosis peaked at 3 months after operation, the expression of type Ⅰ collagen gradually increased after operation, and the expression of MMP-1 was lower in the early stage after angioplasty to 6 Month and reached the normal blood vessel level, and TIMP-1 in the early post-angioplasty was significantly increased, reaching the peak within 3 months, 6 months was still stable at a high level; VSMC phenotype in early is mainly synthetic, and The later stage was mainly contracted. The ratio of TIMP 1 / MMP 1 was positively correlated with neointimal area and type Ⅰ collagen expression (r = 0.56, P <0.01). CONCLUSION: The extracellular type I collagen gradually increases with the passage of time after angioplasty. The dynamic evolution of type I collagen mainly depends on the activity ratio of TIMP 1 / MMP 1, and it is related to the phenotypic characteristics of VSMC