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目的 6-羟基多巴(6-OHDA)两点单侧注射内侧前脑束建立帕金森病(PD)大鼠模型,寻求简单高效的模型制备方法。方法 105只健康雄性SD大鼠随机分为正常对照组(n=10),生理盐水对照组(n=20)和模型组(n=75)。模型组SD大鼠以立体定向两点单侧注射6-OHDA至内侧前脑束。术后不同时间点观察行为学改变,术后1周开始,每周1次腹腔注射阿朴吗啡(Apo)诱发大鼠旋转行为,连续4周,分别计数完全成功大鼠模型只数。此后2周1次Apo诱发大鼠旋转行为,记录转数。结果模型组大鼠最早于术后1周内可出现异常行为姿势。第1、2、3、4周成功模型只数分别为7、23、17、2只。第10周成功模型大鼠的旋转次数与4周前相比有显著差异(P<0.01)。结论单侧内侧前脑束注射6-OHDA诱发大鼠的行为学改变与PD患者临床表现有相似之处。PD大鼠模型诱发旋转次数较为稳定,随损毁时间延长有增加趋势。
OBJECTIVE To establish a rat model of Parkinson’s disease (PD) by injecting 6-OHDA into the medial forebrain bundle at one time, and to seek a simple and efficient model preparation method. Methods 105 healthy male SD rats were randomly divided into normal control group (n = 10), saline control group (n = 20) and model group (n = 75). Model group SD rats were stereotactically injected with 6-OHDA unilaterally to the medial forebrain bundle at two points. Behavioral changes were observed at different time points after operation, starting from 1 week after the operation, apomorphine (Apo) was given intraperitoneally once every week to induce rat rotation behavior for 4 consecutive weeks, and the number of completely successful rat models was counted. Apo-induced rat rotation behavior was performed 2 weeks later, and the number of revolutions was recorded. Results The rats in the model group could have abnormal behavior posture as early as 1 week after operation. The number of successful models at the first, second, third and fourth weeks were 7,23,17,2 respectively. The number of rotations of rats in the 10th week was significantly different from that in the 4th week (P <0.01). Conclusions The behavioral changes of 6-OHDA-induced rats in unilateral medial forebrain bundle are similar to those of PD patients. PD rat model induced more stable rotation times, with the extension of the damage has an increasing trend.