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对毛白杨内生真菌区系种群分析 ,筛选出优势种毛壳ND35 ,并测试了它对 6种常见林果病害病原真菌的拮抗作用。结果表明 ,毛壳ND35是一种广谱性拮抗菌 ,对供试的 6种病菌存在不同程度的抑制作用。可降低病菌菌丝的生长率和孢子的萌发率 ,并对小核菌的菌核进行重寄生 ,从而抑制其萌发。毛壳ND35的菌丝还产生吸器、附着枝等结构对立枯丝核菌 (Rhizoctoniasolani)等病菌的菌丝进行重寄生 ,在显微镜下可观察到病菌的菌丝被缠绕、穿透、断裂等现象。抑菌带和抑菌圈的产生证明抗生活动是毛壳ND35的主要拮抗作用机制之一。对活体毛白杨组培苗进行人工接种试验 ,结果用毛壳ND35预先接种的苗木发病率为零 ,明显低于只接烂皮病菌的对照发病率 5 3 33%,进一步证实了毛壳ND35对植物病害的生物防治潜能。
The analysis of the floristic population of endophytic fungi in Populus tomentosa shows that the dominant species Trichosanthes unguiculatus ND35 was screened and its antagonism to six common species of pathogen fungus was tested. The results showed that the hairy shell ND35 was a broad-spectrum antagonistic bacterium, which inhibited the six pathogens tested to varying degrees. Can reduce the growth rate of mycelium of bacteria and spore germination rate, and the sclerotium of the sclerotinia parasite, thereby inhibiting its germination. The mycelia of the hairy shell ND35 also produce inhalers, attachments and other structures to re-parasitize hyphae of Rhizoctonia solani. The hyphae of the germs are entangled, penetrated and ruptured under a microscope . Antibiotics and inhibition zones proved that antimicrobial activity was one of the major antagonistic mechanisms of the hair cell ND35. The artificial inoculation test of the tissue culture seedlings of Populus tomentosa showed that the incidence of nursery stock pre-inoculated with the wool coat ND35 was zero, which was significantly lower than that of the control with the barely pathogenic bacteria of 5 3 33%, further confirming that the ND35 pair Biological potential of plant diseases.