论文部分内容阅读
目的探讨G-蛋白是否参与了吗啡的舒血管效应。方法用G-蛋白抑制剂百日咳毒素（PTX）孵育血管条8～10h，观察吗啡对血管条的作用。结果吗啡使脱羟肾上腺素（PE）预收缩的血管条舒张，舒张百分数为26.8％±0.4％，较对照明显增强，P＜0.001，使PE预收缩的血管条（经PTX预孵育）舒张;与对照组相比，舒张10min、20min两组之间有显著差异P＜0.05。结论吗啡可抑制PE引起的主动脉血管平滑肌细胞膜受体依赖钙通道（ROC）的开放，阻止外钙内流。吗啡的舒血管效应可能有G-蛋白参与。
Objective To investigate whether G-protein is involved in the vasodilator effect of morphine. Methods The vascular strips were incubated with pertussis toxin (PTX), a G-protein inhibitor, for 8 to 10 hours to observe the effect of morphine on vascular strips. RESULTS Morphine attenuated the pre-contracted vascular strips of phenylephrine (PE) by 26.8% ± 0.4%, significantly higher than that of the control (P <0.001). The pre-contracted vascular strips (pre-incubated with PTX) Compared with the control group, diastolic 10min, 20min significant difference between the two groups P <0.05. Conclusion Morphine can inhibit the opening of receptor-dependent calcium channel (ROC) of aortic vascular smooth muscle cells induced by PE and prevent the influx of extracellular calcium. The vasodilator effect of morphine may have G-protein involvement.