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研究克菌丹致人支气管上皮细胞(16HBE)染色体损伤的情况。在加和不加体外代谢活化系统S9情况下,克菌丹染毒16HBE细胞后,收获不同染毒时相各剂量组的细胞进行染色体畸变分析。在不加S9时,0.0625～1.25mg/L剂量范围内,随染毒剂量的增加,细胞染色体畸变率升高,并呈剂量-反应关系;72h1次染毒、2次染毒和3次染毒的细胞染色体畸变率均显著增高,主要表现为染色体结构畸变,且随着染毒次数增加,引起细胞遗传物质损伤所需的最低浓度就越低;在加S9时,不同染毒次数和剂量组细胞间染色体畸变率均无明显差异。克菌丹可诱导16HBE细胞染色体畸变,具有潜在的致突变性。
To investigate the effect of captan on the chromosome damage of human bronchial epithelial cells (16HBE). In addition and without adding metabolic activation system in vitro S9 circumstances, after captan Dan 16HBE cells were harvested cells from different exposure phases of each dose of chromosome aberration analysis. In the range of 0.0625 ~ 1.25mg / L without S9, the rate of chromosome aberration increased with the dose increasing, and the dose-response relationship was observed; 72h1, 2 and 3 d The chromosome aberration rate of the poisoned cells were significantly increased, which was mainly manifested as chromosomal structural aberration. With the increase of the number of exposures, the lowest concentration of cytosolic material required for injury was lower. At the time of adding S9, There was no significant difference in chromosomal aberration between groups. Captan can induce chromosomal aberration of 16HBE cells and has potential mutagenicity.