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目的:通过建立诱发性大鼠肝癌动物模型,研究用刺参酸性粘多糖(SJAMP)对大鼠诱发性肝癌的干预作用及免疫功能的影响。方法:将雄性Wistar大鼠50只随机均分为5个组,正常对照组、模型组和3个SJAMP干预组(A组,B组和C组),模型组和SJAMP干预组灌胃0.2%DEN生理盐水溶液以诱发肝癌,同时SJAMP干预组按照不同剂量(0.175μg/g,0.35μg/g,0.7μg/g)给予SJAMP,至16周处死大鼠,取血,无菌取脾、胸腺,计算脾指数、胸腺指数。比较各组>3mm和>5mm的结节数以及最大结节的体积,计算肿瘤抑制率。贴壁法纯化巨噬细胞,用中性红吞噬实验检测巨噬细胞吞噬功能,MTT法检测巨噬细胞杀伤功能。结果:SJAMP干预组>3mm和>5mm的结节数明显少于模型组,最大结节的平均体积明显小于模型组(P<0.05);与模型组相比,SJAMP干预组脾指数和胸腺指数明显升高(P<0.05),SJAMP干预组巨噬细胞吞噬能力和杀伤功能显著提高(P<0.05)。结论:刺参酸性粘多糖对大鼠诱发性肝癌有明显的抑制作用;其机制可能是通过刺激免疫器官生长,增强机体的细胞免疫能力来实现的。
OBJECTIVE: To establish an animal model of induced hepatocellular carcinoma (HCC) in rats and study the effects of SJAMP on the induction of hepatocellular carcinoma in rats and its immune function. Methods: Fifty male Wistar rats were randomly divided into five groups: normal control group, model group and three SJAMP intervention groups (group A, group B and group C). The model group and SJAMP intervention group were fed with 0.2% DEN saline solution to induce hepatocellular carcinoma. SJAMP intervention group was given SJAMP at different doses (0.175μg / g, 0.35μg / g, 0.7μg / g), rats were sacrificed at 16 weeks, blood was collected, , Calculated spleen index, thymus index. Comparing the number of nodules> 3mm and> 5mm in each group and the volume of the largest nodule, calculate the tumor inhibition rate. Macrophages were purified by adherent method, macrophage phagocytosis was detected by neutral red phagocytosis, and macrophage cytotoxicity was detected by MTT assay. Results: The number of nodules> 3mm and> 5mm in SJAMP intervention group was significantly less than that in model group, and the mean nodule volume was significantly smaller in model group than in model group (P <0.05). Compared with model group, spleen index and thymus index (P <0.05). The phagocytosis and killing function of macrophages in SJAMP group were significantly increased (P <0.05). CONCLUSION: Antisense polysaccharides of Acanthopanax senticosus can obviously inhibit the induced hepatocarcinoma in rats. The mechanism may be through stimulating the growth of immune organs and enhancing the cellular immunity of rats.