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目的通过检测动脉粥样硬化(AS)患者血液中基质金属蛋白酶-9(MMP-9)与基质金属蛋白酶抑制物-1(TIMP-1)的基因表达水平,探讨AS发生的分子机制及预测因子。方法 AS患者120例为动脉硬化组,72例正常体检者为对照组。用实时荧光定量PCR(RT-PCR)检测其血液中MMP-9和TIMP-1 mRNA表达水平。结果 MMP-9和TIMP-1的RT-PCR产物序列与GenBank上人MMP-9 mRNA和TIMP-1基因片段完全一致。动脉硬化组的MMP-9基因表达水平高于对照组MMP-9基因表达水平,但差异无统计学意义(P>0.05)。动脉硬化组的TIMP-1基因表达水平低于对照组TIMP-1基因表达水平,差异有统计学意义(P<0.05)。MMP-9/TIMP-1基因表达定量比值,动脉硬化组高于对照组,差异有统计学意义(P<0.05)。结论 AS患者TIMP-1基因表达水平的降低与MMP-9/TIMP-1基因表达定量比值升高可能是AS发生的分子机制之一,二者一起可以作为AS发生的预测因子。
Objective To explore the molecular mechanism and predictors of AS by detecting the gene expression of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-1 (TIMP-1) in the blood of patients with atherosclerosis (AS) . Methods 120 patients with AS were atherosclerosis group and 72 normal subjects were control group. The expression of MMP-9 and TIMP-1 mRNA in blood was detected by real-time fluorescence quantitative PCR (RT-PCR). Results The RT-PCR product sequences of MMP-9 and TIMP-1 were completely consistent with the human MMP-9 mRNA and TIMP-1 gene fragments in GenBank. The level of MMP-9 gene expression in atherosclerosis group was higher than that in control group, but the difference was not statistically significant (P> 0.05). TIMP-1 gene expression in atherosclerosis group was lower than that in control group, the difference was statistically significant (P <0.05). The quantitative ratio of MMP-9 / TIMP-1 gene expression in atherosclerosis group was higher than that in control group, the difference was statistically significant (P <0.05). Conclusions The decrease of TIMP-1 gene expression and the quantitative ratio of MMP-9 / TIMP-1 gene expression in AS patients may be one of the molecular mechanisms of AS, which together can be used as a predictor of AS.