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目的 研究P16,P5 3基因在人肺癌中的表达和DNA倍体的关系及意义。方法 以SP免疫组化法检测肺癌组织中P16,P5 3表达 ,图像分析仪测定DNA倍体。结果 P16在肺癌表达阳性率为 5 1.2 % ,低于癌旁组织 (P <0 .0 5 ) ,腺癌P16阳性率最高为 68.8% ,高于鳞癌及未分化癌 (P <0 .0 5 ) ,P5 3在肺癌表达阳性率为 80 .5 % ,明显高于癌旁组织 (P<0 .0 1)其中鳞癌阳性率高于其他组织类型 (P <0 .0 5 ) ,在有淋巴结转移和无淋巴结转移组间及Ⅰ、Ⅱ期与Ⅲ、Ⅳ期两组间 ,P16和P5 3表达阳性率均有显著差异 (P <0 .0 1)。同时发现二倍体P16阳性率 ,P5 3阴性率均显著高于异倍体 (P <0 .0 5 )。结论 P16和P5 3基因异常与肺癌发生有关 ,且在不同类型肺癌发生中的作用不同 ,可作为判断肺癌生物学行为和预后的参考指标。
Objective To study the relationship between the expression of P16 and P53 in human lung cancer and DNA ploidy and its significance. Methods SP immunohistochemical method was used to detect the expression of P16 and P53 in lung cancer tissues. DNA ploidy was detected by image analyzer. Results The positive rate of P16 expression in lung cancer was 52.2%, lower than that in paracancerous tissues (P <0.05). The positive rate of P16 in adenocarcinoma was 68.8%, higher than that in squamous cell carcinoma and undifferentiated carcinoma (P <0. 5). The positive rate of P53 in lung cancer was 80.5%, which was significantly higher than that in adjacent non-cancerous tissues (P <0.01). The positive rate of squamous cell carcinoma was higher than that of other tissues (P <0.05) The positive rates of P16 and P53 between the two groups with lymph node metastasis and no lymph node metastasis, as well as stage I, II and III, IV were significantly different (P <0.01). At the same time, we found that the positive rate of P16 and the negative rate of P53 were all significantly higher than aneuploid (P <0.05). Conclusion The abnormalities of P16 and P53 genes are related to the occurrence of lung cancer and have different roles in different types of lung cancer, which may be used as a reference index to judge the biological behavior and prognosis of lung cancer.