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目的构建基于铂与石墨烯纳米复合物(Pt@GR)的电化学免疫传感器。方法在洁净的玻碳电极表面滴涂一层壳聚糖与Pt@GR的复合物,通过共价作用将纳米金固定在电极表面,进一步固载甲胎蛋白抗体制得甲胎蛋白免疫传感器,采用循环伏安法(cyclic voltammetry,CV)、示差脉冲伏安法(differential pulse voltammetry,DPV)对实验条件进行优化,对该传感器的响应特性进行研究。同时制备基于普鲁士蓝和石墨烯的免疫传感器以对照研究不同传感器的性能特点。结果免疫反应最佳时间为24 min,测试底液最佳p H为6.5。在最优的实验条件下,连续扫描100圈,其电流响应下降5.1%。平行测定5支电极的RSD为8.1%,加标回收率为96.40%~102.49%。在测试底液中加入过氧化氢,其线性范围为0.1~100.0 ng/m L,检测限为0.026 ng/m L。结论基于Pt@GR复合物修饰的电化学传感器具有灵敏度高和稳定性好的特点,可用于甲胎蛋白的高灵敏检测。
Objective To construct an electrochemical immunosensor based on platinum and graphene nanocomposites (Pt @ GR). Methods A layer of complex of chitosan and Pt @ GR was dripped on the surface of a clean glassy carbon electrode. Gold nanoparticles were immobilized on the surface of electrode through covalent reaction. AFP antibody was immobilized on the surface of the clean glassy carbon electrode. The experimental conditions were optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV), and the response characteristics of the sensor were studied. At the same time, immunosensors based on Prussian blue and graphene were prepared to study the performance characteristics of different sensors. Results The best time of immune reaction was 24 min, and the best pH of the test solution was 6.5. Under the optimal experimental conditions, the current response decreased by 5.1% after 100 consecutive sweeps. The RSDs of 5 electrodes in parallel were 8.1% and the recoveries were 96.40% ~ 102.49%. Hydrogen peroxide was added to the test solution with a linear range of 0.1-100.0 ng / mL and a limit of detection of 0.026 ng / mL. Conclusion The electrochemical sensor based on Pt @ GR complex modification has the characteristics of high sensitivity and good stability, which can be used for the high sensitive detection of alpha-fetoprotein.