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为获得纯化心钠素(ANP)单体,采用离子交换及疏水柱层析,纯化融合蛋白麦芽糖结合蛋白(MBP)-ANP和MBP-3ANP,用凝血因子Xa切割MBP-ANP后,经阳离子柱分离获得ANP单体.对ANP单体与BMP-3ANP进行生物学活性检测.1 材料与方法1.1 材料含心钠素多拷贝基因的重组表达质粒pMal-nANP...
In order to obtain purified ANP monomer, the fusion protein maltose binding protein (MBP) -ANP and MBP-3ANP were purified by ion-exchange and hydrophobic column chromatography, MBP-ANP was excised with coagulation factor Xa, Isolated ANP monomer.Animals and BMP-3ANP ANP monomer for biological activity testing.1 Materials and methods 1.1 Materials containing a copy of the atrial natriuretic gene expression plasmid pMal-nANP ...