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目的:探讨复方鳖甲软肝方药物血清对大鼠肝星形细胞(HSC)增殖细胞核抗原(PCNA)表达的影响。方法:运用免疫细胞化学和图像分析技术方法,从离体的角度对不同时相、不同浓度复方鳖甲软肝方药物血清干预状态下,HSC内PC-NA的表达进行半定量分析,观察复方鳖甲软肝方药物血清对离体大鼠HSC增殖的影响。结果:复方鳖甲软肝方药物血清可明显抑制HSC中PCNA的表达。结论:本研究提示复方鳖甲软肝方具有抑制HSC增殖的功能,进而起到抑制肝纤维化发生、发展的作用。
Objective: To investigate the effect of compound Biejia Ruangan prescription serum on the expression of proliferating cell nuclear antigen (PCNA) in rat hepatic stellate cells (HSC). METHODS: Immunocytochemistry and image analysis techniques were used to semi-quantitatively analyze the expression of PC-NA in HSCs with different phases and different concentrations of Compound Biejia Ruangan Recipe serum in vitro. Effect of Biejia Ruangan Prescription Serum on the Proliferation of Isolated Rat HSCs . Results: The serum of compound Biejia Ruangan prescription can obviously inhibit the expression of PCNA in HSC. Conclusion: This study suggests that compound Biejia Ruangan Fang has the function of inhibiting the proliferation of HSC, and then plays a role in inhibiting the occurrence and development of hepatic fibrosis.