论文部分内容阅读
目的观察血府逐瘀汤对肺间质纤维化(PF)模型动物的影响。方法小鼠气管内注射平阳霉素复制PF模型后分为模型组,血府逐瘀汤低(18.75 g/kg)、高(31.25 g/kg)剂量组,设空白对照组,连续灌胃28 d,检测肺组织羟脯氨酸(HYP)以及胶原纤维、弹性纤维含量;大鼠PF模型复制同前,术后分为模型组、血府逐瘀汤(25 g/kg)组、醋酸沷尼松(PA,6.25 mg/kg)组,设空白对照组,连续灌胃28 d,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、层黏连蛋白(LN)及透明质酸(HA)含量的动态变化。结果与空白对照组比较,模型组小鼠肺组织HYP、胶原纤维含量显著增多而弹性纤维含量大幅降低,血府逐瘀汤各组均可减少其HYP、胶原纤维含量,提高弹性纤维含量(P<0.05)。与空白对照组相比,模型组大鼠同期血清SOD活性降低,MDA、LN、HA升高,并有显著性差异(P<0.05);与模型组比较,血府逐瘀汤组大鼠血清MDA、HA含量均不同程度减少,血清SOD活性增加(P<0.05),而对血清LN含量则没有明显影响。结论血府逐瘀汤对PF模型动物的氧自由基损伤有不同程度的干预作用,并通过降低血清HA、肺组织HYP、胶原蛋白含量及提高弹性纤维含量,改善其细胞外基质(ECM)代谢。
Objective To observe the effect of Xuefu Zhuyu Decoction on the animal model of pulmonary interstitial fibrosis (PF). Methods PF mice were injected intratracheally with Pingyangmycin (PF) into trachea model group. The Xuefu Zhuyu Decoction (18.75 g / kg) and high dose (31.25 g / kg) d, The content of hydroxyproline (HYP), collagen fibers and elastic fibers in the lung tissue were detected. The rat PF model was duplicated the same way as the model group, Xuefuzhuyu Decoction (25 g / kg) (PA, 6.25 mg / kg). The rats in the control group were given gavage for 28 days. The levels of serum superoxide dismutase (SOD), malondialdehyde (MDA), laminin (LN) Dynamic changes of HA content. Results Compared with the blank control group, the content of HYP, collagen fibers and the content of the elastic fibers in the model group were significantly decreased, and the contents of HYP and collagen fibers in the Xuefuzhuyu Decoction group were decreased, and the content of the elastic fibers (P <0.05). Compared with the blank control group, serum SOD activity, MDA, LN and HA in the model group were significantly increased at the same period (P <0.05). Compared with the model group, the serum level of serum in the Xuefuzhuyu Decoction group The content of MDA and HA reduced to different degrees, and the activity of serum SOD increased (P <0.05), but had no significant effect on the content of serum LN. Conclusion Xuefu Zhuyu Decoction has different effects on oxygen free radical damage in PF model animals, and can improve ECM metabolism by decreasing serum HA, lung tissue HYP, collagen content and increasing elastic fiber content .