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众所周知,CD_4~+细胞受Ⅱ类MHC 约束,而CD_8~+细胞受Ⅰ类MHC 约束,在此基础上,进一步研究CD_4~+8~+细胞的选择,以阐明CD_4协同受体参与T 细胞的选择。用基因工程建立二个系的LCK-CD_4转基因小鼠,它们的胸腺细胞表达CD_4的量比正常小鼠高15～20倍。αβ转基因小鼠的TCR对H-Y 抗原有特异性,且为H-2D Ⅰ类分子。为确定成熟CD_8~+T 细胞是否受表达CD_4转基因的影响,将CD_4转基因小鼠(727系)与αβ转基因小鼠配对(αβ/727系)。用单抗(抗CD_4或CD_8,α-T3.70或β-F23.1)染色来分析αβ转基因雌鼠及既有αβ又有727
It is well-known that CD_4 ~ + cells are restricted by MHC class II and CD_8 ~ + cells are restricted by MHC class I, on the basis of which we further study the selection of CD_4 ~ +8 ~ + cells to elucidate the role of CD_4 co-receptors in T cells select. Two lines of LCK-CD4 transgenic mice were established by genetic engineering. Their thymocytes express 15 ~ 20 times more CD_4 than normal mice. The TCR of αβ transgenic mice is specific for the H-Y antigen and is a H-2D class I molecule. To determine whether mature CD8 + T cells are affected by the expression of the CD4 transgene, CD4 transgenic mice (line 727) were paired with [alpha] [beta] transgenic mice ([alpha] [beta] / 727 lines). Αβ transgenic female mice were also stained with monoclonal antibodies (anti-CD_4 or CD_8, α-T3.70 or β-F23.1), and both αβ and 727