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枣在成熟期间遇到连阴雨天气会发生严重的裂果现象,这种基于基因表达的生理病害的发生与非生物或生物胁迫有关。以吕梁当地木枣为试材,在木枣成熟期,采用改良CTAB法检测组织中DNA纯度及含量的变化,研究ABA、H2O2与NO供体硝普钠(SNP)在枣果发育中对基因表达的影响。结果表明:ABA、H2O2与NO供体硝普纳(SNP)对枣果发育中DNA纯度及含量的影响不一,差异较为明显。其中不同浓度的ABA处理枣果组织中DNA的OD260/OD280比值基本不在1.700~1.900范围内,DNA纯度差异明显。但样品DNA含量较高,其值在1 200ng/μL左右;H2O2和SNP处理样品DNA的OD260/OD280比值基本上在1.700~1.900之间,DNA纯度较高。样品DNA含量基本上小于或在1 000ng/μL左右。外源ABA较为明显地干扰了基因表达,诱导了新蛋白质、RNA和酚类物质等的生物合成,有促进木枣果发育的趋势;而H2O2和NO供体硝普钠(SNP)对基因表达或枣果发育的影响不十分明显。
Jujube encountered severe raging phenomenon even in rainy days during maturity. The occurrence of physiological diseases based on gene expression is related to abiotic or biological stress. In the mature period of jujube, the changes of DNA purity and content in the tissue were detected by modified CTAB method. The effects of ABA, H2O2 and nitric oxide (NO) donor of NO donor on the development of jujube The impact of expression. The results showed that ABA, H2O2 and NO donor nitroprusside (SNP) had different effects on the purity and content of DNA in jujube fruit development, the difference was obvious. Among them, the OD260 / OD280 ratio of DNA in jujube fruits treated with different concentrations of ABA was basically not within the range of 1.700 to 1.900, and the DNA purity was significantly different. However, the DNA content of the sample was high at a value of about 1 200 ng / μL. The OD260 / OD280 ratio of DNA samples treated with H2O2 and SNP was basically between 1.700 and 1.900, and the DNA purity was high. Sample DNA content is substantially less than or at about 1 000 ng / μL. Exogenous ABA interfered with the gene expression obviously, induced the biosynthesis of new proteins, RNA and phenolic compounds, and promoted the development of Juglans regia. However, the effects of H2O2 and NO donor SNP on gene expression Or jujube development is not very obvious.