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多数学者认为树鼩属低等灵长类动物,关于树鼩局部脑缺血的研究尚未见报导。用电凝阻断树鼩一侧大脑中动脉(MCA-O)的方法复制局部脑缺血模型。发现血管闭塞15分钟及6小时后,缺血区rCBF分别为凝闭前的36.74%和39.84%,SEP分别为凝闭前的21.83%和36.78%;血管闭塞6小时后,皮层组织ATP含量减少了5.2倍,AMP含量增加了4.4倍;钙显著强多,MDA含量增加。形态学检查表明发生了局部缺血性脑梗塞。结果说明了能量衰竭,脂质过氧化物的形成,Ca~(2+)内流增加在脑缺血损伤发病中起重要作用。作者认为,由于树鼩的生物学特性,较其它哺乳动物更适合局部脑缺血的实验研究。
Most scholars believe that tree shrews are of lower primates. No studies have reported on tree shrews with focal cerebral ischemia. The focal cerebral ischemia model was replicated by electrocoagulation to block the middle cerebral artery (MCA-O) of the tree shrew. After 15 minutes and 6 hours of vascular occlusion, the rCBF in the ischemic area was 36.74% and 39.84% before the clot, and the SEP was 21.83% and 36.78% before the clot, respectively; after 6 hours of occlusion, the ATP content in the cortex tissue decreased. At 5.2-fold, the AMP content increased by 4.4 times; calcium was significantly stronger and MDA content was increased. Morphological examination showed that ischemic cerebral infarction occurred. The results demonstrated that energy failure, formation of lipid peroxides, and increase of Ca 2+ influx play an important role in the pathogenesis of cerebral ischemic injury. The authors believe that due to the biological characteristics of tree shrew, it is more suitable for experimental studies of local cerebral ischemia than other mammals.