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以氯化亚铁和氯化铁为原料,通过共沉淀法制备了亲水性的超顺磁性氧化铁纳米粒子(SPION),然后对油酸的羧基进行活化,采用化学方法制备油酸改性超顺磁性氧化铁纳米粒子(O-SPION)。用XRD、FTIR、DLS、TEM对产物结构进行了表征,并通过MTT法检测了O-SPION对人肝癌细胞HepG2的毒性作用,普鲁士蓝染色法检测了其细胞摄取能力。结果表明:合成的O-SPION形态规则,其核心粒径为(12±1.5)nm,Zeta电位为(36±1.5)m V,可在有机溶剂中形成稳定的磁流体。体外细胞实验显示,当O-SPION质量浓度最高为800 mg/L时,O-SPION对HepG2细胞无明显毒性。普鲁士蓝染色图可见,O-SPION较未改性的SPION细胞摄取量明显增多。
Hydrophilic superparamagnetic iron oxide nanoparticles (SPION) were prepared by coprecipitation method using ferrous chloride and ferric chloride as raw materials. Then the carboxyl groups of oleic acid were activated and the oleic acid was modified by chemical method Superparamagnetic iron oxide nanoparticles (O-SPION). The structure of the product was characterized by XRD, FTIR, DLS and TEM. The toxic effect of O-SPION on HepG2 cells was detected by MTT assay. The cell uptake capacity was detected by Prussian blue staining. The results showed that the morphology of O-SPION synthesized was (12 ± 1.5) nm and the zeta potential was (36 ± 1.5) mV. The stable magnetic fluid could be formed in organic solvent. In vitro experiments showed that O-SPION had no obvious toxicity on HepG2 cells when the mass concentration of O-SPION was 800 mg / L. Prussian blue staining shows that, O-SPION SPION unmodified cells significantly increased uptake.