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目的：为探讨非小细胞肺癌中p53蛋白表达与肺癌组织学类型、分化程度和淋巴结转移的关系。方法：应用免疫级化技术（ABC法），以p53单克隆抗体检测53例非小细胞肺癌组织中p53基因的表达，组织经微波抗原修复后进行免疫染色。结果采用X2检验。结果：p53蛋白在53例非小细胞肺癌中表达阳性率为64．2％，p53蛋白表达与非小细胞肺癌的组织学类型无关（P＞0.05）；与肺鳞癌分化程度有关（P＜0.01）；肺腺癌中与淋巴结转移有关（p＜0.05）。结论：p53基因的表达异常与不同类型的肺癌发生发展相关，提示P53基因在肺癌的形成、分化和转移过程中起不同的调节作用，p53蛋白过度表达则预后差，检测p53蛋白对判断非小细胞肺癌预后具有应用价值。
Objective: To investigate the relationship between the expression of p53 protein and the histological type, differentiation and lymph node metastasis of non-small cell lung cancer. METHODS: Immunohistochemical technique (ABC method) was used to detect the expression of p53 gene in 53 cases of non-small cell lung cancer tissues with p53 monoclonal antibody. Tissues were repaired by microwave antigen and immunostained. The result was X2 test. RESULTS: The positive expression rate of p53 protein in 53 cases of non-small cell lung cancer was 64.2%. The expression of p53 protein was not associated with the histological type of NSCLC (P>0.05). It was related to the degree of differentiation of lung squamous cell carcinoma (P< 0.01); Lung adenocarcinoma was associated with lymph node metastasis (p<0.05). Conclusion: The abnormal expression of p53 gene is associated with the development of different types of lung cancer, suggesting that P53 plays a different role in the process of lung cancer formation, differentiation and metastasis. The overexpression of p53 protein has a poor prognosis. The prognosis of cell lung cancer has application value.