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目的:研究芒果叶醇提取物(MLE)对小鼠急性酒精肝损伤的影响。方法:18~22 g昆明种小鼠,雌雄各半,随机分为正常组、模型组、阳性药(护肝片1.5 g·kg-1)组,MLE高、中、低(15.0,5.0,2.5 g·kg-1)剂量组,每组10只。小鼠1次性灌胃50%乙醇(12 mL·kg-1)建立急性酒精性肝损伤模型,测定血清中丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)活性及甘油三酯(TG)含量,检测肝组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)与丙二醛(MDA),并进行肝组织病理切片检查。结果:与模型组比较,MLE高、中、低剂量组血清TG与肝组织MDA含量及肝损伤病理积分均明显降低(P<0.05);MLE高、中剂量组小鼠血清ALT与AST活性明显降低(P<0.05),肝组织GSH水平明显升高(P<0.05);MLE高剂量组肝组织T-SOD活性明显升高(P<0.05)。结论:MLE对小鼠急性酒精肝损伤具有保护作用,可能与其抗脂质过氧化作用相关。
Objective: To study the effect of mango leaf alcohol extract (MLE) on acute alcoholic liver injury in mice. Methods: Kunming mice (18-22 g) were randomly divided into normal group, model group and positive control group (1.5 g · kg-1) 2.5 g · kg-1) dose group, 10 rats in each group. Acute alcoholic liver injury model was established by intragastric administration of 50% ethanol (12 mL · kg-1) in mice once a day, and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and triglyceride (T-SOD), glutathione (GSH) and malondialdehyde (MDA) in liver tissue were measured and the histopathological examination of liver tissue was performed. Results: Compared with the model group, the levels of serum TG, liver MDA and pathological scores of liver injury in MLE high, medium and low dose groups were significantly decreased (P <0.05); the serum ALT and AST activities in MLE high and medium dose groups were significantly (P <0.05). The level of GSH in liver tissue was significantly increased (P <0.05). The activity of T-SOD in liver tissue of high-dose MLE group was significantly increased (P <0.05). Conclusion: MLE has a protective effect on acute alcoholic liver injury in mice, which may be related to its anti-lipid peroxidation.