目的建立微生物法测定药品与食品中叶酸含量的方法,解决液相色谱不能测定食品化合物形式叶酸含量的不足,为孕妇叶酸日摄入量的统计提供理论依据。方法利用酪乳酸杆菌对叶酸生长的依赖性,测定其菌液光密度值,得到叶酸含量。结果检测3个种类的样品,得到了批内、批间、回收率的数据,并与液相色谱的检测结果做了比较。结论微生物法稳定可靠,比液相色谱法应用更广。 OBJECTIVE To establish a method for the determination of folic acid content in pharmaceuticals and foods by microbiological method and to solve the problem that liquid chromatography can not determine the content of folic acid in the form of food compounds and provide a theoretical basis for the statistics of daily intake of folic acid in pregnant women. Methods Dependent on the growth of folate by Lactobacillus casei, the optical density of the strain was measured to get the folic acid content. Results Three kinds of samples were tested, and the data of in-batch, inter-batch and recovery were obtained and compared with the results of liquid chromatography. Conclusion Microbiological method is stable and reliable, which is more widely used than liquid chromatography.