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目的:研究青蒿素(Art)对结状神经节神经元动作电位(AP)的影响,以明确其抗心律失常和麻醉作用的机制。方法:选择新生和成年SD大鼠制备分离神经元和结状神经节-迷走神经切片标本,全细胞膜片箝方法记录AP,切片标本测量感觉传入纤维的传导速率(CV),并以氯胺酮为参照评价Art对AP和CV的作用。结果:Art的作用如下:1) AP除极过程减慢,静息膜电位(RMP)不变,AP幅度和除极速度明显降低(P<0.01),2) 动作电位复极化中点(APD_(50))持续时间延长(P<0.01),3) AP复极过程减慢,复极速度降低,AP超极化程度减弱,4) AP的内向和外向电流明显减小,5) Art不影响CV,6)对AP的影响呈剂量依赖性,并与氯胺酮的作用相似。结论:Art抑制AP的除极和复极过程,此作用可能至少与其阻断Na~+和K~+通道的作用有关。
Objective: To study the effect of artemisinin (Art) on action potential (AP) of nodose ganglion neurons in order to clarify the mechanism of antiarrhythmic and anesthetic effects. METHODS: Neonatal and adult Sprague-Dawley rats were selected to prepare isolated neurons and nodose-vagal nerve sections. Whole-cell patch-clamp technique was used to record AP and slice specimens were used to measure sensory afferent fiber conduction velocity (CV). Ketamine was used as a reference. Evaluate the effect of Art on AP and CV. RESULTS: The effects of Art were as follows: 1) AP depolarization was slowed, resting membrane potential (RMP) was unchanged, AP amplitude and depolarization velocity were significantly reduced (P < 0.01), and 2) action potential repolarization midpoint ( APD_(50)) prolonged duration (P<0.01), 3) AP repolarization slowed down, repolarization speed decreased, AP hyperpolarization decreased, 4) AP inward and outward current decreased significantly, 5) Art Does not affect CV, 6) has a dose-dependent effect on AP and is similar to ketamine. Conclusion: Art inhibits the process of depolarization and repolarization of AP. This effect may be related to at least the role of blocking Na~+ and K~+ channels.