以人的胚胎cDNA为模板 ,用PCR方法筛选获得rab13基因 ,并克隆到真核表达载体pcDNA3和原核表达载体pGEX、pET 15b和 pMAL c2中 .把rab13基因转染入牛肾上腺毛细血管内皮细胞 (BCE细胞 )中 ,免疫荧光染色显示Rab13定位于BCE细胞胞质内膜性细胞器上 .在大肠杆菌DH5a或BL2 1:DE3中表达的原核质粒表达系统均显示rab13基因的高表达 .这些结果为rab13基因对膜通道调节功能的进一步研究打下了基础 The human embryonic cDNA was used as a template and the rab13 gene was screened by PCR and cloned into eukaryotic expression vector pcDNA3 and prokaryotic expression vector pGEX, pET 15b and pMAL c2.The rab13 gene was transfected into bovine adrenal capillary endothelial cells BCE cells), immunofluorescence staining showed that Rab13 was localized in the cytoplasmic membrane organelles of BCE cells.The prokaryotic plasmid expression system expressed in E. coli DH5a or BL21: DE3 showed the high expression of rab13 gene.These results were rab13 Gene laid the foundation for further study of membrane channel regulation function