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[目的]探讨肝细胞癌患者病灶局部Treg细胞的变化规律及其临床意义。[方法]收集40例原发性肝细胞型肝癌(HCC)患者经手术切除的肝癌组织及非癌肝组织(距肿瘤边缘>5cm)标本,用流式细胞仪分别检测肝癌组织、非癌肝组织中调节性T淋巴细胞(Treg细胞)的数量及变化规律,并且分析Treg细胞表达数量与预后的关系。[结果]肝癌病灶局部有不同程度免疫细胞浸润,主要为T淋巴细胞,其中Treg淋巴细胞含量高于非肿瘤组织。Ⅲ、Ⅳ期患者Treg细胞含量(7.7%±4.03%)明显高于Ⅰ、Ⅱ期患者(3.7%±1.6%)(P=0.037)。高Treg细胞含量组(≥6%)患者的预后明显差于低Treg细胞含量组(<6%)(P=0.005)。[结论]肝癌组织中,对免疫起抑制作用的Treg细胞比例增加。Treg细胞含量高与预后差相关。
[Objective] To investigate the variation of local Treg cells in patients with hepatocellular carcinoma and its clinical significance. [Methods] 40 cases of primary hepatocellular carcinoma (HCC) were collected from patients with hepatocellular carcinoma (HCC) and non-cancerous liver tissues (> 5 cm from the edge of the tumor). Flow cytometry was used to detect the expression of HCC, Treg cells in the number of regulatory T cells and changes in law, and analyze the relationship between the number of Treg cells and prognosis. [Results] There were some degree of immune cell infiltration in HCC lesions, mainly T lymphocytes, and the content of Treg lymphocytes was higher than that in non-tumor tissues. The content of Treg cells in stage Ⅲ and Ⅳ (7.7% ± 4.03%) was significantly higher than that in stage Ⅰ and Ⅱ (3.7% ± 1.6%) (P = 0.037). Patients with high Treg cell content (> 6%) had significantly worse outcomes than those with low Treg cell content (<6%) (P = 0.005). [Conclusion] The proportion of Treg cells that have an inhibitory effect on immunity increased in HCC tissues. High Treg cell content correlates with poor prognosis.