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目的:采用纤维素酶提取法和超声提取法提取中药复方补气升血方药中的有效成分,探讨两种提取方法对该方药药物药效的影响。方法:定期对小鼠进行灌胃,利用小鼠酚红排泌实验、炭粒廓清法实验计算炭粒廓清指数K值、吞噬指数α值,二甲苯致小鼠耳肿胀实验比较不同组小鼠的耳肿胀度,从而比较两种提取液的药效学差异。结果:两种提取液均可以减轻小鼠的耳肿胀度、升高廓清指数K值和吞噬指数α值,但纤维素酶提取法作用优于超声提取法。结论:纤维素酶提取法提取补气升血方药药效优于超声提取法。
OBJECTIVE: To extract the effective constituents of traditional Chinese medicine Buqi Shengqi Recipe by using cellulase extraction and ultrasonic extraction, and to explore the effects of two extraction methods on the efficacy of this recipe. Methods: The mice were gavaged on a regular basis. The mice were excreted with phenol red excretion, and the carbon clearance index (K), the phagocytic index (α) and the ear edema induced by xylene were compared between different groups of mice Of the ear swelling degree, so as to compare the pharmacological differences between the two extracts. Results: Both extracts could relieve ear swelling in mice and increase the clearance index K and phagocytic index α, but the effect of cellulase extraction was better than that of ultrasonic extraction. CONCLUSION: Cellulase extraction is superior to ultrasound in extraction of Qi-Qi Sheng-Xie prescription.