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目的探讨联合接种多种肺炎球菌热休克蛋白(heat shock proteins,HSPs)对小鼠抵抗肺炎球菌能力的影响。方法将3种肺炎球菌HSPs Clp P、Dna J、Gro EL单一及经任意组合后分别经腹腔免疫小鼠,每次免疫接种间隔12~14 d,共接种3次。末次免疫7 d后,经小鼠眼眶采血,分离血清,测定各组小鼠血清抗体滴度,同时设佐剂对照组(AlPO_4)。将单一蛋白免疫组小鼠经鼻腔黏膜分别感染中剂量肺炎球菌2/D39(1.0×10~7 CFU)、3/CMCC31436(3.0×10~7 CFU)、4/TIGR4(2.0×10~7 CFU),所有免疫组小鼠均经鼻腔黏膜分别感染致死剂量肺炎球菌2/D39(2.0×10~8 CFU)、3/CMCC31436(1.0×10~9 CFU)、4/TIGR4(5.0×10~8 CFU),比较各组小鼠的中位生存时间。结果单一蛋白及联合蛋白免疫组小鼠均可产生有效抗体。单一蛋白免疫组小鼠感染3种中等剂量肺炎球菌后的中位生存时间均明显长于佐剂对照组(P<0.001);联合蛋白免疫组小鼠感染不同致死剂量菌株肺炎球菌后的中位生存时间明显长于单一蛋白免疫组(P<0.05)。结论联合接种多种肺炎球菌HSPs可提高小鼠抵抗肺炎球菌能力,为其作用机制的研究奠定了基础。
Objective To investigate the effects of heat shock proteins (HSPs) inoculated with various pneumococci on mice resistance to pneumococci. Methods Three kinds of pneumococcal HSPs, Clp P, Dna J and Gro EL, were immunized intraperitoneally and singly and intraperitoneally. The mice were immunized intraperitoneally for 12-14 days with 3 immunizations. Seven days after the last immunization, blood was collected from the orbit of mice and the serum was separated. The antibody titers of the mice in each group were determined. At the same time, adjuvant control group (AlPO_4) was established. The single immunized mice were infected with moderate dose pneumococcal 2 / D39 (1.0 × 10-7 CFU), 3 / CMCC31436 (3.0 × 10-7 CFU) and 4 / TIGR4 (2.0 × 10-7 CFU) ), And all mice in the immunized group were infected with lethal doses of pneumococcal 2 / D39 (2.0 × 10-8 CFU), 3 / CMCC31436 (1.0 × 10-9 CFU) and 4 / TIGR4 (5.0 × 10-8) CFU) to compare the median survival time of mice in each group. Results A single protein and the combination of protein immunized mice can produce effective antibodies. The median survival time of single protein immunized mice infected with three medium dose pneumococci was significantly longer than that of adjuvant control group (P <0.001). The combined protein immunized mice had the median survival after infection with different lethal doses of pneumococcus The time was significantly longer than the single protein immunization group (P <0.05). Conclusions The combined inoculation of a variety of pneumococcal HSPs can enhance the ability of mice to resist pneumococci, and lay a foundation for its mechanism of action.