目的 :建立反相高效液相色谱同时测定来氟米特及A77172 6的方法。方法 :采用HypersilODSC18柱 ,乙腈-醋酸盐缓冲液为流动相 ,检测波长分别为 2 6 0nm及 2 90nm ,流速 1 40mL·min-1,进样量 2 0 μL ,肉桂酸为内标。结果 :能同时测定来氟米特及A77172 6 ,来氟米特线性范围 0 79～ 31 6 0 μg·mL-1(r =0 9999) ,平均回收率为 10 0 3% (RSD =0 76 % ) ;A77172 6线性范围 0 35～ 14 0 0 μg·mL-1(r =0 9996 ) ,平均回收率为 99 6 9%(RSD =0 95 % )。结论 :本法简便、快速、准确 Objective: To establish a method for simultaneous determination of leflunomide and A77172 6 by reversed-phase high performance liquid chromatography. METHODS: Hypersil ODSC18 column and acetonitrile-acetate buffer were used as the mobile phase. The detection wavelength was 260 nm and 290 nm respectively. The flow rate was 140 mL · min-1. The injection volume was 20 μL and the cinnamic acid was used as internal standard. Results: The leflunomide and A77172 6 were measured at the same time. The linear range of leflunomide was 0 79 ~ 31 60 μg · mL-1 (r = 0 9999) with the average recovery of 10 0 3% (RSD = 0 76 %); A77172 6 linear range of 0 35 ~ 14 0 0 μg · mL-1 (r = 0 9996), the average recovery was 99 6 9% (RSD = 0 95%). Conclusion: This method is simple, fast and accurate