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目的:观察登革2型病毒衣壳蛋白(D2C)与葡萄球菌核酸酶(SN)融合蛋白在哺乳动物细胞中对登革2型病毒增殖的影响。方法:通过基因重组构建可在哺乳动物细胞中表达融合蛋白D2C-SN的重组质粒pc/D2C-SN,同步构建pc/D2C-SN*用作对照。病毒感染BHK21细胞后,通过阳离子转染试剂将重组质粒导入感染细胞,在此基础上评价融合蛋白D2C-SN抗登革病毒感染的治疗效果。结果:融合蛋白D2C-SN能够在哺乳动物细胞中表达,对宿主细胞没有明显的毒性;与正常BHK21细胞相比较,可导致病毒感染性滴度降到原来的1/60~1/12。结论:融合蛋白D2C-SN在细胞水平能够有效抑制登革病毒的增殖,有可能成为潜在的抗登革病毒感染的治疗性药物。
Objective: To observe the effect of Dengue virus type 2 capsid protein (D2C) and staphylococcal nuclease (SN) fusion protein on the proliferation of Dengue 2 virus in mammalian cells. METHODS: Recombinant plasmid pc / D2C-SN was constructed by gene recombination to express the fusion protein D2C-SN in mammalian cells. Simultaneous construction of pc / D2C-SN * was used as a control. After infection with BHK21 virus, the recombinant plasmid was transfected into infected cells by cationic transfection reagent, and then the therapeutic effect of fusion protein D2C-SN against dengue virus infection was evaluated. Results: The fusion protein D2C-SN could express in mammalian cells without obvious cytotoxicity to host cells. Compared with normal BHK21 cells, the infectious titer of D2C-SN could be reduced from 1/60 to 1/12. Conclusion: The fusion protein D2C-SN can effectively inhibit the proliferation of dengue virus at the cellular level and may become a potential therapeutic drug against dengue virus infection.