论文部分内容阅读
为选育矮化和耐缺铁的苹果砧木,配置了小金海棠×M9杂交组合,首先筛选M9纯合显性SSR分子标记并利用该标记鉴定小金海棠×M9的有性杂种后代,之后利用流式细胞术分析杂交后代中的倍性分离。结果表明:SSR标记CH02d12-160可作为鉴定杂种后代的特异标记。在1 285株小金海棠×M9实生苗中,有284株为有性杂种后代,其余1 001株为小金海棠无融合生殖后代,无融合生殖率为77.90%。在284株小金海棠×M9有性杂种后代中,138株的样品得到可靠倍性鉴定结果,其中2x、3x、4x和5x分别为5(3.62%)、21(15.22%)、63(45.65%)和49株(35.51%)。
In order to select the dwarf and iron-deficient apple rootstocks, the hybrid combination of Xiaojinhaitang × M9 was screened. First, the M9 homozygous dominant SSR marker was screened and the sexual hybrid progenies of Xiaoguxi × M9 were identified by this marker. Cytometry analysis of ploidy in hybrid progeny. The results showed that SSR marker CH02d12-160 could be used as a specific marker to identify offspring of hybrids. Among the 1 285 plants of M. officinalis × M9, 284 were sexual hybrid offspring, and the remaining 1 001 were progeny of apomixis. The rate of apomixis was 77.90%. The results of reliable ploidy identification showed that among 2x2, 3x, 4x and 5x, 138 (45.2%) were 21 (15.22%) and 21 (15.22% ) And 49 strains (35.51%).