In 2010,cytidine 5'-triphosphate synthase (CTPS) was reported to form the filamentous or serpentine structure in Drosophila,which we termed the cytoophidium.In the last decade,CTPS filaments/cytoophidia have been found in bacteria,budding yeast,human cells,mice,fission yeast,plants,and archaea,indicating that this mechanism is highly conserved in evolution.In addition to CTPS,other metabolic enzymes have been identified to have the characteristics of forming cytoophidia or similar advanced structures,demonstrating that this is a basic strategy of cells.Nevertheless,our understanding of the physiological function of the cytoophidium remains incomplete and elusive.Here,we took the larva of Drosophila melanogaster as a model to systematically describe the localization and distribution of cytoophidia in different tissues during larval development.We found that the distribution pattern of CTPS cytoophidia is dynamic and heterogenic in larval tissues.Our study provides a road map for further understanding of the function and regulatory mechanism of cytoophidia.