尽管可以得到高亲和力放射活性标记配体,但纯化盐皮质激素受体(MR)一直非常困难。作者利用一含有编码人糖皮质激素受体(hGCR)DNA结合区的 cDNA 片段(hGCR_(1.2),1100bp)从人胎盘 DNA 中筛选出-2.5Kb 的 Hind Ⅲ片段,用 HindⅢ消化人胎盘 DNA,用分离到的2.5Kb 的 DNA 片段构建一基因文库,再以 hGCR_(1.2)为探针,筛选到一 Despite the availability of high affinity radioactive labeled ligands, it has been very difficult to purify the mineralocorticoid receptor (MR). The authors screened a HindIII fragment of -2.5 kb from human placental DNA using a cDNA fragment (hGCR (1.2), 1100 bp) encoding the DNA binding region of the human glucocorticoid receptor (hGCR), digested human placental DNA with HindIII, A 2.5kb DNA fragment was used to construct a gene library, and then hGCR_ (1.2) was used as a probe to screen a