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家鼠脑中线粒体(MIT)和微粒体(MIC)中芳香烃羟化酶(AHH)活性研究是多功能的氧化酶(MFO)系统的典型例子.MIT的AHH需要N-ADH,而MIC的AHH需要NADPH,NADH和N-ADPH作为酶活性最佳表达时电子供给者。以苯(a)芘(BP)为底物,MIT的AHH其Km值为1.18μM,MIC的AHH之Km值为6.66μM.MIT和MIC的AHH皆对氰化物敏感,而酶活性的抑制表现剂量依赖性.作者发现MIC同MIT的AHH一样能用3-甲-胆蒽(3-MC)和苯巴比妥(PB)诱导,但单一PB只引起MIC的AHH轻微诱导,对
Aromatic hydroxylase (AHH) activity studies in mitochondria (MIT) and microsomes (MICs) in domestic mice are typical examples of multifunctional oxidase (MFO) systems. AHH for MIT requires N-ADH whereas MIC AHH requires NADPH, NADH and N-ADPH as electron providers for optimal expression of enzyme activity. Using benzene (a) pyrene (BP) as substrate, the KH value of AHH of MIT was 1.18 μM and the MIC value of AHH of MIC was 6.66 μM. The AHH values of MIT and MIC were all sensitive to cyanide, while the inhibition of enzyme activity The authors found that MICs can be induced with 3-methyl-chrysene (3-MC) and phenobarbital (PB) just as MIT’s AHH, but only a single PB induces mild AHH induction of MICs,