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血管内皮细胞(EC)的重要性不仅在于它是血液和血管壁之间的屏障,还在于它可分泌多种物质对血管张力,血管平滑肌细胞生长,血小板功能和凝血机制产生调节作用。血管内皮损伤是多种疾病,特别是心血管疾病的基础,而缺氧是引起EC 功能障碍的常见原因。一氧化氮(NO)和内皮素(ET)是血管内皮分泌的重要活性物质,是血管张力和血小板功能的重要调节因子。穿心莲有效成分—API(API_(0134))既有抗血小板活化作用,也有促进内皮纤溶活性作用,但对 EC 在缺氧条件下是否具有保护作用还不清楚。本研究目的在于观察其对缺氧培养的人脐静脉内皮细胞 NO、ET 合成和超微结构的影响,并与抗血小板药物噻氯匹啶(ticlopidine,TP))进行比较。
The importance of vascular endothelial cells (EC) is not only that it is a barrier between the blood and blood vessel walls, but also that it secretes various substances that regulate vascular tone, vascular smooth muscle cell growth, platelet function, and coagulation mechanisms. Vascular endothelial injury is the basis of many diseases, particularly cardiovascular diseases, and hypoxia is a common cause of EC dysfunction. Nitric oxide (NO) and endothelin (ET) are important active substances for vascular endothelium secretion and are important regulators of vascular tone and platelet function. The active ingredient of Andrographis paniculata (API_(0134)) has both anti-platelet activation and endothelial fibrinolytic activity, but it is unclear whether EC has a protective effect under hypoxic conditions. The purpose of this study was to observe the effects of hypoxia on the synthesis and ultrastructure of human umbilical vein endothelial cells (HUVECs), and to compare them with the antiplatelet drug ticlopidine (TP).