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目的:分析扶正化瘀方(Fuzheng Huayu decoction,FZHY)灌胃大鼠后进入血液的五味子中木脂素类成分,以探讨部分体内效应成分。方法:应用液相色谱-电喷雾质谱联用(HPLC-ESI-MSn)技术,通过空白血清,口服FZHY方后含药血清,FZHY方和对照品之间的色谱图和质谱碎片信息的对比分析,结合文献报道,确定口服FZHY方后大鼠的入血成分。结果:FZHY方和口服FZHY方后含药血清中均含有五味子醇甲、五味子醇乙、五味子酯甲、五味子乙素,并在FZHY方中还发现含有五味子甲素和丙素。结论:五味子醇甲、五味子醇乙、五味子酯甲、五味子乙素是直接吸收入血的成分,可能是发挥抗肝纤维化的体内效应成分。
OBJECTIVE: To analyze the content of lignans in the blood of Fuzheng Huayu decoction (FZHY) fed into rats and explore the effect of some components in vivo. Methods: The HPLC-ESI-MSn technique was used to compare the chromatograms and mass spectral fragments between the serum containing FZHY and the FZHY prescription and the reference substance , Combined with the literature to determine the oral composition of rats after oral FZHY prescription. Results: Both FZHY prescription and oral FZHY prescription contained Schisandrin A, Schisandra B, Schisandra B and Schisandrin B, and FZHY prescription and FZHY prescription contained deoxyschizandrin and prunella. Conclusion: Schisandra alcohol A, Schisandra B, Schisandra B, Schisandrin B is a direct absorption into the blood components, may play an anti-liver fibrosis in vivo components.