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目的 :为了观察血清中TNF α、IL 8水平与慢性病毒性肝炎的关系。方法 :采用ELISA双抗体夹心法检测TNF α、IL 8。结果 :慢性肝炎轻度 ,慢性肝炎中度及慢性重型肝炎血清中TNF α、IL 8水平与对照组相比均有显著性差异 (P <0 0 5～ 0 0 1)。慢性肝炎中度血清中TNF α、IL 8水平明显高于慢性肝炎轻度 (P <0 0 5 )。慢性重型肝炎血清中TNF α、IL 8水平明显高于慢性肝炎轻度及慢性肝炎中度 (P <0 0 5 )。TNF α、IL 8水平的变化趋势与ALT ,TBil水平的变化趋势呈正相关。各组患者TNF α含量的变化与IL 8含量的变化呈正相关。慢性重型肝炎存活组血清TNF α、IL 8水平明显低于慢性重型肝炎死亡组 (P <0 0 5 ) ,重叠感染慢性肝炎患者血清中TNF α、IL 8水平明显高于单纯病毒感染组 (P <0 0 5～ 0 0 1)。结论 :TNF α、IL 8在慢性肝炎中互相影响 ,并在慢性肝炎炎症损伤中起重要作用 ,且可作为判断肝脏受损程度及判断预后的一种指标
Objective: To observe the relationship between TNFα and IL-8 in serum and chronic viral hepatitis. Methods: TNF α and IL 8 were detected by ELISA double antibody sandwich method. Results: The levels of TNFα and IL-8 in serum of moderate and chronic severe hepatitis patients with mild and chronic hepatitis were significantly different from those in control group (P <0.05-0.01). The serum levels of TNFα and IL-8 in chronic hepatitis were significantly higher than those in chronic hepatitis (P <0.05). Serum levels of TNFα and IL-8 in chronic severe hepatitis were significantly higher than those in mild and chronic hepatitis with chronic hepatitis (P <0.05). The changes of TNFα and IL-8 levels were positively correlated with the changes of ALT and TBil levels. The changes of TNFα in each group were positively correlated with the changes of IL 8. Serum levels of TNFα and IL-8 in patients with chronic severe hepatitis were significantly lower than those in patients with chronic severe hepatitis (P <0.05). The levels of TNFα and IL-8 in patients with chronic hepatitis were significantly higher than those in patients with viral infection <0 0 5 ~ 0 0 1). CONCLUSION: TNFα and IL-8 affect each other in chronic hepatitis and play an important role in the inflammatory injury of chronic hepatitis, and can be used as an index to judge the degree of liver damage and prognosis