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为了探讨丙型肝炎病毒（ＨＣＶ）感染的基因型及其临床意义，本文对沈阳地区６１例ＨＣＶ－ＲＮＡ阳性血清的聚合酶链反应（ＰＣＲ）产物，用限制性片段长度多态性（ＲＦＬＰ）分析进行基因分型，结果为：ＨＣＶⅡ型感染占７０．５％（４３／６１），ＨＣＶⅢ型感染占２７．９％（１７／６１），ＨＣＶⅡ／Ⅲ型混合感染占１．６％（１／６１）。初步证明沈阳地区ＨＣＶ感染以Ⅱ型为主。对９１例肝癌病人中ＨＣＶ－ＲＮＡ阳性的２７例血清基因分型，Ⅱ型占７４％（２０／２７），其余为Ⅲ型。可见ＨＣＶⅡ型感染与肝癌发生有密切关系。
In order to investigate the genotype of hepatitis C virus (HCV) infection and its clinical significance, the polymerase chain reaction (PCR) products of 61 HCV-RNA positive sera in Shenyang were used for restriction fragment length polymorphism (RFLP). Genotyping was performed for analysis. The results were: HCV type II infection accounted for 70.5% (43/61), HCVIII type infection accounted for 27.9% (17/61), and HCV type II/III type combined infection accounted for 1.6% (1 /61). It was initially demonstrated that HCV infection in Shenyang is dominated by type II. Among the 91 cases of liver cancer patients, 27 cases of HCV-RNA positive were genotyped, type II accounted for 74% (20/27), and the rest were type III. HCV type II infection is closely related to the occurrence of liver cancer.