论文部分内容阅读
目的观察~(32)P-磷酸铬-聚L-乳酸(~(32) P-CP-PLLA)粒子植入及~(32) P-CP注射后对荷人前列腺癌裸鼠移植瘤的治疗效应。方法建立雄性BALB/c裸小鼠荷人前列腺癌细胞株PC-3M皮下移植瘤模型,随机分为三组,每组15只。设为对照组、~(32) P-CP(胶体组)和~(32) P-CP-PLLA(粒子组)。每组各5只裸鼠,每4天测量肿瘤体积,第28天处死小鼠,测量瘤体质量、微血管密度(MVD)和瘤内残余放射性活度。另每组各10只裸鼠定期监测生命指征,采用Kalplan-Meier曲线分析生存情况。结果给药后第28天,粒子组瘤内剩余活度高于胶体组[(1.74±0.~(32))MBq vs.(1.01±0.43)MBq](P<0.01)。粒子组抑瘤率高于胶体组[(59.24±5.68)%vs.(49.52±6.35)%](P<0.01)。粒子组的MVD低于胶体组和对照组(~(32).24±10.07vs.43.15±11.06和62.71±8.21)(P<0.01)。结论 ~(32)P-CP-PLLA粒子植入可以抑制肿瘤生长和血管生成。
Objective To observe the effects of (32) P-chromate-poly-L-lactic acid (~ (32) P-CP-PLLA) implantation on the xenografted human prostate cancer xenografts in nude mice after ~ (32) P- effect. Methods A subcutaneous xenograft model of human prostate cancer cell line PC-3M in BALB / c nude mice was established and randomly divided into three groups of 15. The control group, ~ (32) P-CP (colloidal group) and ~ (32) P-CP-PLLA (particle group) Five nude mice in each group were used to measure the tumor volume every four days. The mice were sacrificed on the 28th day and the tumor mass, microvessel density (MVD) and residual radioactivity in the tumor were measured. Another 10 nude mice in each group were monitored for vital signs on a regular basis and Kalplan-Meier curves were used to analyze the survival. Results On the 28th day after administration, the residual activity in the intragranular group was higher than that in the colloidal group [(1.74 ± 0.32) MBq vs. (1.01 ± 0.43) MBq] (P <0.01). The tumor inhibition rate of the particle group was higher than that of the colloid group [(59.24 ± 5.68)% vs (49.52 ± 6.35)%] (P <0.01). The MVD of the particle group was lower than that of the colloid group and the control group (~ (32) .24 ± 10.07 vs. 43.15 ± 11.06 and 62.71 ± 8.21) (P <0.01). Conclusion ~ (32) P-CP-PLLA can inhibit tumor growth and angiogenesis.