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目的 探讨质子磁共振波谱 ( 1H -MRS)对帕金森病 (Parkinson’sdisease ,PD)的诊断价值。方法 对 2只恒河猴经左侧颈内动脉注射 1-甲基 4-苯基 1,2 ,3 ,6-四氢吡啶 (MPTP)制备偏侧PD模型前后的双侧基底节区进行 1H -MRS检测 ,对比分析制模术前和术后猴双侧基底节区N -乙酰基天门冬氨酸 (NAA) /肌酸复合物 (Cr)和胆碱复合物 (Cho) /Cr比值的变化 ,并应用透射电镜观察制模术后猴纹状体的超微结构变化。结果 2只猴均成功制备成偏侧PD模型。偏侧PD模型猴MPTP注射侧对侧基底节区NAA/Cr和Cho/Cr比值较制模术前无明显改变 (Ρ >0 .0 5 ) ;但偏侧PD模型猴MPTP注射侧基底节区NAA/Cr比值较制模术前明显降低 ,也明显低于对侧 (Ρ <0 .0 5 ) ;Cho/Cr比值较制模术前明显增高 ,也明显高于对侧 (Ρ <0 .0 5 )。偏侧PD模型猴MPTP注射侧纹状体的超微结构呈神经元细胞线粒体肿胀和空泡化、粗面内质网和高尔基复合体囊腔扩张、脂褐素增多和星形胶质细胞增生等病理性改变 ,但MPTP注射侧对侧纹状体的超微结构未发现异常改变。结论 1H -MRS是可以为偏侧PD模型猴基底节区的神经细胞病理学改变提供有价值的信息的一种无创技术 ,有助于PD的诊断。
Objective To investigate the diagnostic value of proton magnetic resonance spectroscopy (1H-MRS) in Parkinson’s disease (PD). Methods Bilateral basal ganglia before and after preparation of hemiparkinsonism PD model were prepared by injecting 1-methyl-4-phenyl-1,2,4,6-tetrahydropyridine (MPTP) into the left internal carotid artery -MRS were used to detect the ratios of NAA / Cr and Cho / Cr in the bilateral basal ganglia of pre-and post-operation monkeys The ultrastructural changes of the monkey striatum were observed by transmission electron microscopy. Results All the two monkeys were successfully prepared into PD model. The ratio of NAA / Cr and Cho / Cr in the contralateral basal ganglia of MPTP injection in the hemiparkinsonian PD model was not significantly different from that in the model MPTP (P> 0.05), but the hemiparkinsonism The NAA / Cr ratio was significantly lower than that before modeling (P <0.05), and the Cho / Cr ratio was significantly higher than that before modeling (P <0. 0 5). The ultrastructure of striatum on the side of MPTP injection in the hemiparkinsonian PD model showed swelling and vacuolization of mitochondria in neuronal cells, cystic dilatation of rough endoplasmic reticulum and Golgi complex, lipofuscin increase and astrocyte proliferation And other pathological changes, but there was no abnormal change in the ultrastructure of contralateral striatum on the MPTP injection side. Conclusion 1H-MRS is a noninvasive technique that can provide valuable information for neuropathological changes in the basal ganglia of hemiparkinsonian PD model, which is helpful for the diagnosis of PD.