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采用多聚酶链反应（ＰＣＲ）技术检测水中肠道病毒前，肠道病毒应先行浓集。本研究成功地用金葡菌固相抗体对水中肠道病毒的指标菌ｆ＿２噬菌体进行浓集。（１）金葡菌固相抗体先行混悬于水样中，与ｆ＿２噬菌体结合，然后在３０００ｒ／ｍｉｎ下离心１０ｍｉｎ。１ｍｌ金葡菌固相抗体约可吸附３０００ＰＦＵ以上的ｆ＿２噬菌体。（２）蒸馏水、ＮａＣｌ溶液、磷酸盐缓冲液（ＰＢＳ）及三倍肉汤对金葡菌固相抗体吸附ｆ＿２的效果均无明显影响，吸附率均在９３．７％以上。（３）用ＰＣＲ技术对金葡菌固相抗体捕获的脊髓灰质炎病毒Ｉ型疫苗株（ＰＶＩ）亦可检测。
Before using polymerase chain reaction (PCR) to detect waterborne enterovirus, enterovirus should be concentrated first. In this study, Staphylococcus aureus solid-phase antibody was successfully used to collect the indicator phage f_2 phage of enterovirus in water. (1) Staphylococcus aureus solid-phase antibody was first suspended in water and bound to f 2 bacteriophage, then centrifuged at 3000 r / min for 10 min. 1ml Staphylococcus aureus solid phase antibody can adsorb more than 3000PFU phage f_2. (2) The effects of distilled water, NaCl solution, phosphate buffered saline (PBS) and triple broth on the adsorption of f 2 by Staphylococcus aureus solid-phase antibody had no obvious effect. The adsorption rates were above 93.7%. (3) The poliovirus type I vaccine strain (PVI) captured by Staphylococcus aureus solid phase antibody by PCR can also be detected.