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目的 观察不同强度恒磁场对过氧化氢 (H2 O2 )作用下人脐静脉内皮细胞 (HUVEC)增殖与凋亡的影响。方法 采用体外培养第 3代的HUVEC ,实验分为 6组 ,即对照组、1 0 0 μmol/LH2 O2 组及H2 O2 +不同强度 (1Gs、5Gs、1 0Gs、50Gs)磁场组。各组细胞于培养及磁场作用 48h后收集标本 ,用MTT比色法测定细胞增殖 ,末端脱氧核糖核酸酶介导的dUTP末端标记法 (TUNEL法 )和流式细胞仪碘化丙啶染色法检测细胞凋亡。结果 1 0 0μmol/LH2 O2 可抑制HUVEC增殖 ,诱导HUVEC凋亡 (P <0 0 5vs对照组 )。 1Gs、5Gs和 1 0Gs恒磁场组细胞增殖显著高于H2 O2 组 (P <0 0 5) ,细胞凋亡显著低于H2 O2 组 (P <0 0 5) ;50Gs恒磁场组细胞增殖和凋亡与H2 O2 组相比无明显差异 (P >0 0 5)。结论 1 0 0 μmol/LH2 O2 可抑制HUVEC增殖并诱导HUVEC凋亡 ;1～ 1 0Gs的恒磁场可强度依赖性拮抗H2 O2 对HUVEC的作用 ,促进HUVEC增殖并抑制HUVEC凋亡。
Objective To observe the effects of different intensity constant magnetic fields on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H2O2). Methods The third generation of HUVECs were cultured in vitro. The experiment was divided into 6 groups: control group, 100 μmol / LH2 O2 group and H2O2 + 1Gs, 5Gs, 1 0Gs, 50Gs magnetic field group. The cells were harvested at 48 h after culture and magnetic field. Cell proliferation was measured by MTT colorimetric assay, TUNEL assay and flow cytometry propidium iodide staining Apoptosis. Results 100 μmol / LH2 O2 could inhibit the proliferation of HUVEC and induce the apoptosis of HUVEC (P <0.05 vs control group). The cell proliferation of 1Gs, 5Gs and 10Gs constant magnetic field group was significantly higher than that of H2O2 group (P <0.05), and the apoptosis was significantly lower than that of H2O2 group (P <0 05) There was no significant difference between H2O2 group and H2O2 group (P> 0.05). CONCLUSION: 1 000 μmol / LH 2 O 2 can inhibit the proliferation of HUVEC and induce the apoptosis of HUVEC. The constant magnetic field of 1 ~ 10 Gs antagonizes the effect of H 2 O 2 on HUVEC in an intensity-dependent manner and promotes the proliferation of HUVEC and inhibits the apoptosis of HUVEC.