Ophiolites represent fragments of ancient oceanic lithosphere, tectonically incorporated into continental margins during plate subduction or remained in the subduction-collisional orogenic belt. We report for the first time a Paleoproterozoic supra-subduction zone maficultramafic complex from north Huangling dome, northern Yangtze craton. They are dismembered and incorporated within a NE distributed mélange belt, and show close association with the strong deformed biotite-plagioclase gneiss/schist, metapelite, siliceous rocks and granitic gneiss. The metamafic-ultramafic rocks mainly consist of serpentinization harzburgite, olive pyroxenite, and amphibolite. Serpentinization harzburgite shows high magnesium and low silicon characteristic with low SiO_2 content(37.35-38.03 wt.%), high MgO content(37.00-37.96 wt.%) and high Mg~# value(87.5-88.4), ∑REE=3.17-7.91 ppm. They have slightly enriched LREE patterns with flat shape of HREE and MREE distribution, while spinels from the harzburgite show abyssal peridotites origin, indicating a LREE-enriched abyssal residual mantle peridotites affected by subduction metasomatism. The TiO_2 contents of the amphibolite range from 1.02 wt.% to 1.39 wt.%, MgO from 5.87 wt.% to 7.29 wt.%, Mg~# value from 45.9 to 53.0, and Na_2O/K_2O from 2.25 to 2.70,FeO T/MgO from 1.59 to 2.12, indicate that the amphibolite belongs to tholeiite series. Amphibolite exhibit subparallel and generally flat to right-sloping patterns with weak enriched LREE relative to HREEs and enrichment in LILE(e.g. K,Rb, Ba, Th, Cs) along with HFSE negative anomalies such as Nb, Ti, suggest a supra-subduction zone(SSZ) environment with an enriched mantle source for the amphibolite. LA-ICP-MS zircon U-Pb dating give approximate formation ages of 2142-2148 Ma for Amphibolite and a later metasomatic event(2048-2025 Ma) both for amphibolite and serpentinization harzburgite caused by melt/fluid derived from the subducted slab. We infer that the protolith of amphibolite may derived from depleted mantle at early paleoproterozoic(ca. 2.20-2.15 Ga), and zircon in harzburgite show negative eHf(t) values and model age old as Archean, indicate the zircon grains mainly formed in the process of metasomatism by subduction melt/fluid in the mantle wedge. The quartz monzonitic dyke crosscutting the harzburgite yields a crystallization age of 1999±10 Ma, represent the minimum emplacement age of the mafic-ultramafic rocks. In conclusion, we infer that the metamafic-ultramafic rocks is paleoproterozoic(ca. 2.2-2.1 Ga) back arc ophiolite fragments formed in a supra-subduction zone in the northern Huangling dome, Yangtze craton, and it provides important geological evidence for the paleoproterozoic oceanic-continental subduction and accretion-collision orogenic event associated with the amalgamation of the Columbia supercontinent. Ophiolites represent fragments of ancient oceanic lithosphere, tectonically incorporated into continental margins during plate subduction or remained in the subduction-collisional orogenic belt. We report for the first time a Paleoproterozoic supra-subduction zone maficultramafic complex from north Huangling dome, northern Yangtze craton. are dismembered and incorporated within a NE distributed mélange belt, and show close association with the strong deformed biotite-plagioclase gneiss / schist, metapelite, siliceous rocks and granitic gneiss. The metamafic-ultramafic rocks mainly consist of serpentinization harzburgite, olive pyroxenite, and amphibolite . Serpentinization harzburgite shows high magnesium and low silicon characteristic with low SiO 2 content (37.35-38.03 wt.%), High MgO content (37.00-37.96 wt.%) And high Mg ~ # value (87.5-88.4), ΣREE = 3.17 -7.91 ppm. They have slightly enriched LREE patterns with flat shape of HREE and MREE distribution, while spinels from the harzburg ite show abyssal peridotites origin, indicating a LREE-enriched abyssal residual mantle peridotites affected by subduction metasomatism. The TiO 2 contents of the amphibolite range from 1.02 wt.% to 1.39 wt.%, MgO from 5.87 wt.% to 7.29 wt.%, Mg ~ # value from 45.9 to 53.0, and Na2O / K2O from 2.25 to 2.70, FeOT / MgO from 1.59 to 2.12, indicate that the amphibolite belongs to tholeiite series. Amphibolite exhibit subparallel and generally flat to right-sloping patterns with weak enriched LREE relative to HREEs and enrichment in LILE (eg K, Rb, Ba, Th, Cs) along with HFSE negative anomalies such as Nb, Ti, suggest a supra-subduction zone (SSZ) environment with an enriched mantle source for the amphibolite. LA-ICP-MS zircon U-Pb dating give approximate formation of 2142-2148 Ma for Amphibolite and a later metasomatic event (2048-2025 Ma) both for amphibolite and serpentinization harzburgite caused by melt / fluid derived from the subducted slab. We infer that the protolith of amphibolite m ayderived from depleted mantle at early paleoproterozoic (ca. 2.20-2.15 Ga), and zircon in harzburgite show negative eHf (t) values ​​and model age old as Archean, indicate that the zircon grains mainly formed in the process of metasomatism by subduction melt / fluid in the mantle wedge. The quartz monzonitic dyke crosscutting the harzburgite yields a crystallization age of 1999 ± 10 Ma, represent the minimum emplacement age of the mafic-ultramafic rocks. In conclusion, we infer that the metamafic-ultramafic rocks is paleoproterozoic (ca. 2.2-2.1 Ga) back arc ophiolite fragments formed in a supra-subduction zone in the northern Huangling dome, Yangtze craton, and it provides important geological evidence for the paleoproterozoic oceanic-continental subduction and accretion-collision orogenic event associated with the amalgamation of the Columbia supercontinent.