用苹果诱果病难养菌(Fastidious bacteria)制备抗血清包被富含A蛋白的金黄色葡萄球菌。包被菌与苹果锈果病难养菌进行共凝集试验(SA-test),产生沉淀即谓协同共凝集反应(Co-agglutination)。用此法检测了不同来源、不同品系及不同树龄的苹果、巴梨、鸭梨、二十世纪梨。对难养菌接种的实验树也进行了检测。病树样品表现明显的协同共凝集反应,健树样品则无此反直。检测难养菌接种的实验树也证实了回接的成功。讨论了该方法作为苹果锈果病早期快速简便诊断的可靠性,以及应用于生产实际中的可行性。 Antisera were coated with protein A-rich Staphylococcus aureus using apple-induced Fastidious bacteria. Co-agglutination was detected by co-agglutination test (SA-test) between the coated bacteria and the hard-supporting bacteria of the apple rust fruit. This method was used to test different sources, different strains and different ages of apple, pear, pear, twentieth century pear. The experimental tree for challenged vaccination was also tested. Sick tree samples showed a significant co-coagulation reaction, Jianshu samples are not anti-straight. The experimental tree that tested the hard-to-challenge inoculation also confirmed the success of the callback. The feasibility of this method as a quick and easy diagnostic method for early detection of apple rust and the feasibility of its application in production were discussed.