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目的:去卵巢大鼠脑内MDA水平、GSH-Px活性及NO浓度的变化及半枝莲黄酮(Scutellaria Barbata flavonoid,SBF)对这些变化的影响。方法:3月龄SD雌性大鼠摘除卵巢10个月后,用分光光度法检测大脑皮质和海马中丙二醛(malonyldialde-hyde,MDA)、一氧化氮(nitric oxide,NO)的含量及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活性,并观察SBF对这些变化的影响;结合雌激素倍美力为阳性对照药。结果:与假手术组大鼠相比,双侧卵巢摘除大鼠大脑皮质和海马MDA水平分别增加了0.58倍和0.50倍、GSH-Px活性分别降低了40.13%和57.53%,NO含量分别降低了49.25%和44.90%。然而去卵巢大鼠连续灌胃SBF 17.53,57,0 mg.kg-1 36天可不同程度地改善去卵巢大鼠上述神经异常变化,0.065 mg.kg-1倍美力也表现出类似的作用。结论:双侧卵巢摘除可以引起大鼠脑内MDA、NO水平与GSH-Px活性异常改变,而SBF可以对抗上述异常变化,提示SBF这种神经保护作用有利于神经退行性疾病的治疗。
Objective: To investigate the effects of Scutellaria Barbata flavonoid (SBF) on the changes of MDA level, GSH-Px activity and NO concentration in the brain of ovariectomized rats. METHODS: After 3 months of removal of the ovary from SD female rats aged 3 months, the contents and valleys of malondialde-hyde (MDA) and nitric oxide (NO) in the cerebral cortex and hippocampus were measured by spectrophotometry. The activity of glutathione peroxidase (GSH-Px) was observed, and the effect of SBF on these changes was observed; the conjugated estrogen Premarin was a positive control drug. RESULTS: Compared with sham-operated rats, the levels of MDA in cerebral cortex and hippocampus increased 0.58-fold and 0.50-fold respectively, and GSH-Px activity decreased 40.13% and 57.53%, respectively. 49.25% and 44.90%. However, the continuous intragastric administration of SBF 17.53,57,0 mg.kg-1 to ovariectomized rats for 36 days could improve the above-mentioned neurological abnormalities in ovariectomized rats to varying degrees, and the 0.065 mg.kg-1 preservative also showed a similar effect. Conclusion: Bilateral ovariectomy can cause abnormal changes of MDA, NO levels and GSH-Px activity in brain of rats, and SBF can counteract these abnormal changes, suggesting that this neuroprotective effect of SBF is beneficial to the treatment of neurodegenerative diseases.