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本文报近了以胡杨的无菌幼叶作为外植体材料，诱导产生愈伤组织，进而分化形成无菌苗的方法，采用第一轮试管苗的幼叶作外植体比用茎切段作外植体繁殖率高，且由于芽长成的嫩技较小，可以大大节省容器，比茎切段更为经济实用．
This paper reported near Populus sterile young leaves as explant material, inducing the callus, and then differentiated into sterile seedlings using the first round of young shoots of young plant explants than the use of stem segments Reproductive rate of explants is high, and because buds grow into tender technology is small, can greatly save the container, cut more than the stem is more economical and practical.