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目的探讨中胚叶特异性转录子(又名父源表达基因-1)(MEST)的基因印迹在非小细胞肺癌发生发展中的作用。方法利用聚合酶链反应(PCR)技术结合限制性片段长度多态性(PFLP)技术,分析32例非小细胞肺癌及其对应的癌周正常肺组织中MEST基因的表达及其印迹状态。结果 11例MEST杂合子信息样本中,9例(81.8%)肺癌组织发生了印记缺失(LOI),其中6例为低等级、低发展阶段的肿瘤,而与之对应的癌周组织除1例为弱的双等位基因表达之外,均为单等位基因表达。同时11份MEST杂合子信息样本肺癌组织中MEST的平均表达水平是癌旁组织的1.5倍,且二者表达量差异有显著性(P<0.01)。结论 MEST基因的印迹缺失参与了非小细胞肺癌的发生发展过程。
Objective To investigate the role of MES gene in the development of non-small cell lung cancer (NSCLC). Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PFLP) were used to analyze the expression of MEST gene and its imprinting status in 32 non-small cell lung cancer and its corresponding normal lung tissues. Results Of 11 MEST heterozygous information samples, 9 cases (81.8%) of lung cancer tissues had imprinted deletions (LOIs), of which 6 were low-grade, low-stage tumors, while the corresponding peritumoral tissues were excluded For the weak dual allele expression, are single allele expression. At the same time, the average expression level of MEST in 11 MEST heterozygous samples was 1.5 times higher than that in adjacent non-cancerous tissues, and the difference was significant (P <0.01). Conclusion The imprinting deletion of MEST gene is involved in the development of non-small cell lung cancer.