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对复杂化合物的分离除采用高价、精密的仪器之外,用薄层层析法亦能提供分离的手段。定量方法则根据原位置测定或从层析谱上洗脱下组份的溶液进行测定。原位置测定一般是利用化合物的一些物理性质,如光的反射或透射,荧光激发或放射性,采用扫描的方法来完成的。对于反射值测定,在Pye Unican样品装载器上的可变深度的槽,每当使用最浅的深度(2mm)时,也需耗去过多的样品。为此,用铝制成一种插入器,可将样品直径从24mm减至1mm,这样具有减少稀释数量到少于五分之一的作用,每个槽大约可装载100mg硅胶G吸附剂。在校正和测定中,将点于薄板上已知数
The separation of complex compounds in addition to the use of high-priced, sophisticated instruments, the use of thin-layer chromatography can also provide a means of separation. Quantitative methods are based on the determination of the original position or chromatographic eluation of the components of the solution were measured. In situ determination of the general use of some of the physical properties of compounds, such as light reflection or transmission, fluorescence excitation or radioactivity, the use of scanning method to complete. For reflectance measurements, the variable depth slot on the Pye Unican sample loader consumes too much sample each time the shallowest depth (2 mm) is used. To this end, an inserter made of aluminum was used to reduce the sample diameter from 24 mm to 1 mm, thus reducing the amount of dilution to less than one-fifth, each containing approximately 100 mg of silica G adsorbent. In the calibration and determination, the number of points on the plate known