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论述了用桔青霉PenicilliumcitrinumM71菌株 ,经液体培养制得的 5′ -磷酸二酯酶降解鲐鱼鱼精DNA成5′ -脱氧核苷酸的分离工艺。分离采用 2 0 1× 8阴离子交换树脂 ,具体条件为 :柱床高 1 0 5mm ,柱床直径 45mm ,样品浓度 2 1 3mg·mL-1,洗脱流速 0 .5mL·cm-2 ·min-1。分离结果表明 ,采用 0 .0 0 5MHCl+0 .0 4MNaCl作洗脱剂 ,流速为 0 .7mL·cm-2 ·min-1时 ,四种 5’ -脱氧核苷酸组分能完全被洗脱下来 ,且呈一个大峰 ,同其它成分分开 ,再先后采用 0 .0 0 1 8MHCl、0 .0 0 2 8MHCl、0 .0 36MNaCl(pH6 .0 )、0 .0 0 5MHCl+0 .0 2MNaCl作洗脱剂时 ,则能分别将dCMP、dAMP、TMP、dGMP完全分离
This paper discusses the separation of 5 ’- deoxynucleotide from fish carp sperm DNA by liquid culture of Penicillium citrinum M71 by 5’ - phosphodiesterase. The separation was carried out with 2 0 1 × 8 anion exchange resin under the following conditions: column height 150 mm, column diameter 45 mm, sample concentration 21 3 mg · mL -1, elution flow rate 0.5 mL · cm -2 · min -1, 1. The separation results showed that when the flow rate was 0.7 mL · cm-2 · min-1, the four 5’-deoxynucleotide fractions could be completely washed using 0. 05M HCl + 0. 4M NaCl as the eluent Off, and showed a large peak, separated from the other components, followed by 0 .018MHCl, 0.0208MHCl, 0.36MNaCl (pH6.0), 0.050MHCl +0.02MNaCl As eluent, respectively, dCMP, dAMP, TMP, dGMP complete separation