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目的：探讨无氧和／或低氧（０％Ｏ２和／或２５％Ｏ２）对肺动脉内皮细胞（ＰＡＥＣ）活力和增殖的影响。方法：应用形态学观察、细胞活力测定、流式细胞技术、３Ｈ－ＴｄＲ掺入、免疫组化染色等方法。结果：缺氧使ＰＡＥＣ胞浆内的线粒体和粗面内质网增多，随缺氧时间的延长，线粒体肿胀、空泡化，内质网扩张。ＭＴＴ法细胞活力测定显示无氧６ｈ末ＰＡＥＣ的细胞活力无明显变化，无氧１２ｈ至２４ｈ末ＰＡＥＣ的细胞活力明显增加（Ｐ＜００１）。在６ｈ，１２ｈ，１８ｈ，２４ｈ，低氧和无氧使ＰＡＥＣ的３Ｈ－ＴｄＲ掺入显著少于常氧对照组（Ｐ＜００５）。无氧２４ｈ末ＰＡＥＣ的增殖细胞核抗原（ＰＣＮＡ）阳性反应颗粒明显少于常氧对照组，且常氧组ＰＣＮＡ含量是无氧组的３７５倍。然而流式细胞分析显示，与常氧对照组相比，无氧和低氧ＰＡＥＣ的Ｓ期和Ｇ２／Ｍ期细胞比例明显增多（Ｐ＜００１）、Ｇ０／Ｇ１期细胞比例明显减少（Ｐ＜０．００１），细胞内总蛋白除无氧１２ｈ组外含量均显著增多（Ｐ＜０．００１）。结论：缺氧可激活ＰＡＥＣ使其细胞活力增加，但却抑制其增殖；缺氧ＰＡＥＣ的Ｇ２／Ｍ期细胞增多，可能由于缺氧通过延长细胞周期时间和使ＰＡＥＣ的Ｇ２／Ｍ期细胞和Ｇ?
Objective: To investigate the effects of anaerobic and / or hypoxic (0% O2 and / or 2 5% O2) on pulmonary artery endothelial cells (PAEC) activity and proliferation. Methods: Morphological observation, cell viability assay, flow cytometry, 3H-TdR incorporation, immunohistochemical staining and other methods were used. Results: Hypoxia increased mitochondria and rough endoplasmic reticulum in the cytoplasm of PAEC. With the prolongation of hypoxia, mitochondria were swollen, vacuolized and endoplasmic reticulum was dilated. MTT assay showed that there was no significant change in cell viability of PAEC at 6h after anaerobic treatment, and the viability of PAEC increased significantly from 12h to 24h after anaerobic (P <001). At 6h, 12h, 18h, 24h, 3H-TdR incorporation of PAEC was significantly less than that of normoxic control (P <005) under hypoxia and anaerobic conditions. The number of positive proliferating cell nuclear antigen (PCNA) positive particles in PAEC was significantly less than that in normoxic control group at the end of anaerobic 24h, and the PCNA content in normoxia group was 3.75 times higher than that in anaerobic group. However, flow cytometry analysis showed that the proportion of cells in S phase and G2 / M phase increased significantly (P <001) and the proportion of cells in G0 / G1 phase decreased significantly compared with normoxia control group (P < P <0.001). The content of total protein in the cells increased significantly (P <0.001) except for 12h without oxygen. CONCLUSION: Hypoxia can activate PAEC to increase its cell viability, but inhibit its proliferation. The number of G2 / M phase cells in hypoxic PAEC increased, which may be due to prolonged cell cycle time and PA / ?