论文部分内容阅读
目的:研究早期吸入不同浓度布地奈德对哮喘大鼠气道炎症和气道重构的干预情况。方法:32只Wistar大鼠随机分为4组:A对照组8只,B卵蛋白(OVA)致哮喘组8只,C卵蛋白致哮喘后吸入低浓度布地奈德治疗组8只,D卵蛋白致哮喘后吸入高浓度布地奈德治疗组8只。分别测定各组大鼠血中肿瘤坏死因子-α(TNF-α)及肺泡灌洗液(BALF)中内皮素-1(ET-1)的水平,计数BALF中细胞总数及分类。各组大鼠行肺组织切片HE染色,再行胶原染色、免疫组化NGF、TGF-β1染色,借助计算机图象分析软件测量单位气道面积炎性细胞数目,基底膜周径(Pbm)、平滑肌面积(WAm)、气道内壁面积(WAi)、胶原面积(Wcol),NGF及TGF-β1阳性信号积分吸光度。结果:B组BALF中细胞总数、嗜酸细胞分类及TNF-α、ET-1水平与A组比较均明显增加,差异有统计学意义(P<0.01),C组及D组较B组均明显降低,差异有统计学意义(P<0.01)。B组NGF及TGF-β1的表达、气道壁炎性细胞计数、气道内壁面积、平滑肌面积、胶原面积与A组比较均明显增加,差异有统计学意义(P<0.01),C组及D组与B组比较均明显降低,差异有统计学意义(P<0.01),C组及D组与A组比较差异有统计学意义(P<0.05,P<0.01),C组与D组差异均有统计学意义(P<0.05,P<0.01)。结论:早期吸入不同浓度的布地奈德均可明显抑制气道炎症和气道重构,高浓度较低浓度对气道炎症和气道重构的影响更明显。
OBJECTIVE: To study the effects of early inhalation of budesonide on airway inflammation and airway remodeling in asthmatic rats. Methods: Thirty-two Wistar rats were randomly divided into 4 groups: A control group 8, B ovalbumin (OVA) induced asthma 8, C ovalbumin induced asthma after inhalation of low concentration budesonide treatment group 8, D Eight protein-induced budesonide injections were induced in asthmatic rats. Tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1) in bronchoalveolar lavage fluid (BALF) were measured in each group, and the total number and classification of cells in BALF were counted. The lung tissue sections of rats in each group were stained with hematoxylin and eosin and stained with collagen. Immunohistochemical staining of NGF and TGF-β1 was performed. The numbers of inflammatory cells per unit airway area, peritumoral diameter (Pbm) Smooth muscle area (WAm), airway wall area (WAi), collagen area (Wcol), NGF and TGF-β1 positive signal integral absorbance. Results: The total number of cells, eosinophil classification and the levels of TNF-α and ET-1 in BALF were significantly increased in group B compared with group A (P <0.01), and those in group B and group B were significantly higher than those in group B Was significantly lower, the difference was statistically significant (P <0.01). The expression of NGF and TGF-β1, airway wall inflammatory cell count, airway wall area, smooth muscle area and collagen area in group B were significantly increased compared with group A, the difference was statistically significant (P <0.01) There was significant difference between group D and group B (P <0.01), group C and group D were significantly different from group A (P <0.05, P <0.01), group C and group D The differences were statistically significant (P <0.05, P <0.01). Conclusion: Early inhalation of budesonide at different concentrations can significantly inhibit airway inflammation and airway remodeling. The effect of higher concentration and lower concentration on airway inflammation and airway remodeling is more obvious.