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目的检测老年人膀胱移行细胞癌组织中Ｐ１６、Ｐ５３、ｂｃ１２及ｃｅｒｂＢ２的基因表达，探讨其与膀胱癌病理分级、临床分期和转移的关系。方法应用免疫组化ＳＰ法检测５５例老年人膀胱癌组织中Ｐ１６、Ｐ５３、ｂｃ１２及ｃｅｒｂＢ２的基因表达。结果５５例膀胱癌中Ｐ１６、Ｐ５３、ｂｃ１２及ｃｅｒｂＢ２的阳性率分别为４３６％、４９１％、４１８％及４１８％，多种癌基因激活和抑癌基因失活与肿瘤病理分级、临床分期和有无转移有密切的关系。７０９％的肿瘤有上述癌基因和（或）抑癌基因的异常表达，其中５０９％的肿瘤同时有多个基因的表达异常。结论肿瘤的多基因分析比单基因分析更有价值，癌基因ｂｃ１２、ｃｅｒｂＢ２和抑癌基因Ｐ１６、Ｐ５３的表达异常及协同作用可能在老年人膀胱癌的发生、发展中起重要作用。
Objective To detect the gene expression of P16, P53, bc12 and cerbB2 in transitional cell carcinoma of the urinary bladder and to explore its relationship with pathological grade, clinical stage and metastasis of bladder cancer. Methods Immunohistochemical SP method was used to detect the gene expression of P16, P53, bc12 and cerbB2 in 55 elderly patients with bladder cancer. Results The positive rates of P16, P53, bc12 and cerbB2 in 55 cases of bladder cancer were 436%, 491%, 418% and 418%, respectively. A variety of oncogene activation and Inactivation of tumor suppressor gene and tumor pathological grade, clinical stage and metastasis are closely related. 70.9% of the tumors had the above abnormal expression of oncogenes and / or tumor suppressor genes, of which 50.9% of the tumors had abnormal expression of multiple genes at the same time. Conclusions Multi-gene analysis of tumors is more valuable than single-gene analysis. Abnormal expression and synergistic effects of oncogenes bc1-2, c-erbB-2 and tumor suppressor genes P16 and P53 may play an important role in the occurrence and development of bladder cancer in the elderly Important role.