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建立肺孢子虫病动物模型是研究肺孢子虫病原和病理机理的重要手段。目前,国内建立肺孢子虫病动物模型多采用地塞米松诱发自然感染。但此法有较大局限性,它不能用于肺孢子虫保种;不能用无肺孢子虫感染的动物(无特异病原动物)建立感染度一致的肺孢子虫病动物模型;实验动物易中途死亡;不能保证实验动物全部感染。为此,国外学者以鼻腔接种或手术切开气管感染动物。鼻腔接种常有肺孢子虫未达肺部使感染失败。切开气管方法复杂,手术创伤及术后感染易造成动物死亡。本实验采用气管内注入法,可弥补上述方法的不足。
The establishment of animal models of pneumocystosis is to study the pathogens and pathogenic mechanism of Pneumocystis an important means. At present, the domestic establishment of animal models of pneumocystosis use dexamethasone induced natural infection. However, this method is more limited, it can not be used for the maintenance of Pneumocystis; you can not use Pneumocystis-infected animals (non-specific pathogenic animals) to establish an identical degree of infection in animal models of Pneumosporosis; experimental animals easy way Death; can not guarantee that all experimental animals infected. To this end, foreign scholars to nasal or surgical tracheotomy inoculated animals. Pneumocystis often fails to reach the lungs due to nasal inoculation. Tracheotomy complicated method, surgical trauma and infection can easily lead to animal deaths. In this experiment, tracheal injection method can make up for the lack of the above method.